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Steps and methods of microscope illumination

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Steps and methods of microscope illumination

Date of release:2019-07-29 Author: Click:

1. Adjust the direction of the microscope and light source so that the light source is aligned with the microscope's flat collector mirror, but at least 10 inches away. Coal and rock analysis


2. Adjust the light so that the filament can be clearly focused on the flat concentrator.


3. Adjust the mirror so that light is transmitted from the center of the concentrator to the concentrator under the stage.


4. Place a slide on the stage, and move the concentrator down and align the slide specimen, focusing from low to high.


5. Focus clearly on the slide specimen. It may be necessary to increase the concentrating mirror slightly to get enough illumination.


6. Turn the aperture on the light source to a minimum. Carefully screw up (or down) the condenser lens under the stage until the reduced aperture is focused on the same plane as the slide. Do not focus on the microscope itself at this stage (ie, do not mobilize the knob above the stage).

7. If your microscope has the function of a concentrated condenser, you can adjust the aperture to the center of the field of view by moving the image of the aperture (most of the microscopes you can use must do this to move the aperture to the field of view). Central location).


8. Open the aperture ring until the aperture ring of the light polygon fills the field of view.


9. If possible, remove an eyepiece. Close the iris diaphragm of the concentrator under the stage and look into the tube to observe the lens. At this point the light should appear symmetrical. In the research microscope, we can adjust for any asymmetry at this stage.


10. Open or close the iris diaphragm on the concentrator under the small stage, only 2/3 of the field of view of the objective lens. Put back the eyepiece. At this point, the system calibration is completed, and the microscope can be used for microscopic examination of the slide.


11. At low magnification, medium magnification and oil lens use, some compromises must be made. In the microscope you use, this compromise is to adjust the height of the condenser. You must adjust the light intensity by moving the condenser below the correct height. Think about it, what will you sacrifice when you make this compromise?


Do not control the light intensity by moving the concentrator, adjusting the iris aperture on the concentrator under the stage, or enhancing and dimming the light. The ideal control method is to use a neutral filter (ie gray filter).


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